Scripties UMCG - Rijksuniversiteit Groningen
English | Nederlands

Differential distribution of immune cells in decidua basalis and decidua parietalis after uncomplicated term pregnancy, a pilot study

(2017) Laskewitz, A. (Anna)

During pregnancy tolerance towards a fetus needs to be created by the maternal immune
system to prevent a rejection response. Maladaptation of the maternal immune system is
associated with pregnancy complications like preeclampsia, gestational diabetes mellitus and
infertility. The placenta and fetal membranes are important locations where the maternal
immune system needs to create tolerance towards the fetus. Different immune cells like Tcells
and macrophages are known to be located in these tissues and found to be important
during pregnancy. This study aims to analyze maternal tissue from the placenta (decidua
basalis) and maternal tissue from the fetal membranes (decidua parietalis) to see whether there
is a differential distribution of immune cells at these locations.
Materials and methods
The isolation of T-cells and macrophages consisted of a mechanical step and an enzymatic
step. First, maternal and fetal tissue were separated to analyze only maternal tissue. Next, both
tissues were meshed and with an enzymatic solution (Accutase) cells were separated from
both tissues. Using flow cytometry, subsets of T-cells and macrophages were analyzed in
decidua basalis and decidua parietalis. Decidual tissues were compared using a two-way
Higher amounts of different subsets of T-cells were found in decidua parietalis as compared
to decidua basalis. In particular, higher percentages of regulatory T and memory cells were
found in decidua parietalis as compared to decidua basalis. A more regulatory phenotype of
macrophages was found in decidua basalis as compared to decidua parietalis.
Differences between decidua basalis and decidua parietalis were found for different subsets of
T-cells and macrophages. Using comprehensive and up-to-date antibody panels new
knowledge about the maternal immune cells in uncomplicated term pregnancies is brought

To top