Scripties UMCG - Rijksuniversiteit Groningen
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Targeted Gold Nanoparticles for the Computed Tomography Detection of Breast Cancer

(2017) Mubarak-Gabrian, M. (Mariam)

Introduction: As a response to the need for earlier detection of malignant tissues and
metastases, functional imaging has been gaining interest over structural imaging. Gold has
proven to be an attractive alternative to conventional contrast agents, with a higher atomic
number and a higher absorption coefficient compared to iodine. Due to these properties, gold
nanoparticles (AuNPs) could offer a 3-fold increase in contrast per unit weight compared to
iodine-based contrast agents, as well as a better safety profile compared to iodine or
Gadolinium-based agents in terms of renal and cutaneous toxicity.
Methods: Breast cancer cells MDA-MB-231, macrophages RAW 264.7 and fibroblasts MC
3T3.E1 were scanned using a pre-clinical CT to analyze gold nanoparticle uptake. The cells
were analyzed after incubation with a AuNPs solution for four hours and silver staining using
light microscopy.
Results: Breast cancer cells showed the most intense signal for 2-DG-AuNPs (916 HU), and
2-NH2-DG-AuNPs (435 HU), with almost no signal registered for the bare AuNPs, at 22 HU
as opposed to 19 HU for the control sample.
Conclusion: Functionalized 2-DG-AuNPs were a valid targeting contrast agent for cell lines
MDA-MB-231 and RAW 264.7, exhibiting high radiointensity upon CT imaging and low

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